Which layers of the cornea can NOT regenerate? (Choose 2)

The layers of the cornea that are notably unable to regenerate are Bowman's layer and the Endothelium.

Bowman's layer is a tough, acellular layer situated between the corneal epithelium and the stroma. While the epithelium is capable of rapid regeneration in response to injury, Bowman's layer does not have regenerative properties. If it is damaged, it does not heal or reform; instead, a scar may develop, impacting the cornea’s clarity and function.

The Endothelium is the innermost layer of the cornea and plays a critical role in maintaining corneal transparency by regulating fluid within the stroma. Unlike epithelial cells, endothelial cells do not regenerate. If endothelial cells are lost due to pathological conditions or trauma, they cannot be replaced, leading to potential swelling and opacity of the cornea.

Understanding the regenerative capacity of these layers is essential when discussing corneal health, injury, and surgical interventions.